Follow with quantitative analysis, which is highly dependent on the final image quality.Detect the resulting light signal by exposure to X -ray film or c harge-coupled device (CCD) imaging.Add the substrate to membrane - substrates are available in various strengths and stabilities.As with colorimetric detection, the HRP and AP enzymes are used with appropriate substrates: In chemiluminescent detections, the enzyme conjugated to the secondary antibody triggers a reaction with a luminescent substrate that p roduces light as a by-product. Monitor the development of color closely to obtain the best image.
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